Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3770

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Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda}: Lytic Infections on Agar Plates

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

A lytic infection by a recombinant bacteriophage is established in soft agarose. Following induced expression, the recombinant fusion protein is recovered from the infected cells.


MATERIALS

E. coli strain Y1090hsdR

This strain is available from the ATCC (www.atcc.org) and is maintained on LB-agar plates containing 50 µg/ml ampicillin.

Bacteriophage {lambda}gt11 recombinant

Prepare a stock of recombinant bacteriophage {lambda} of known titer by soaking an individual plaque in approx. 100 µl of SM for at least 2 hours at room temperature or by . . . [Full Text of this Article]


METHOD


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