Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3767

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Removal of Cross-reactive Antibodies from Antiserum: Incubation with E. coli Lysate

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes how antibodies that react with bacterial-encoded proteins may be removed from polyclonal antisera by incubation with a bacterial lysate.


MATERIALS

E. coli strain Y1090hsdR

This strain can be obtained from Stratagene, Life Technologies, or the ATCC . . . [Full Text of this Article]


METHOD


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