Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3490

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Purification of Radiolabeled Oligonucleotides by Precipitation with Ethanol

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

If radiolabeled oligonucleotides are to be used only as probes in hybridization experiments, then complete removal of unincorporated radiolabel is generally unnecessary. However, to reduce background, it is always advisable to separate the bulk of the unincorporated radioactivity from the oligonucleotide. Most of the residual radioactive precursors can be removed from the preparation by differential precipitation with ethanol if the oligonucleotide is more than 18 . . . [Full Text of this Article]


MATERIALS


METHOD


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