Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3284

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Large-scale Preparation of Single-stranded and Double-stranded Bacteriophage M13 DNA

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

This protocol, a scaled-up version of Preparation of Double-stranded (Replicative Form) Bacteriophage M13 DNAand Preparation of Single-stranded Bacteriophage M13 DNA, is used chiefly to generate large stocks of double-stranded DNA of strains of M13 that are routinely used as cloning vectors. Large amounts of single-stranded DNA of an individual recombinant may occasionally be needed for specific purposes, e.g., to generate many preparations of a particular radiolabeled probe or to construct large numbers of site-directed mutants.


MATERIALS

E. coli F' plating bacteria

Plating bacteria may be prepared in the laboratory as described in Plating Bacteriophage M13or purchased from commercial . . . [Full Text of this Article]


METHOD


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