Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4481

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Darlington, G. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Darlington, G. J.
Related Collections
Right arrow Virus
Right arrow Molecular Biology, general
Right arrow DNA Delivery/Gene Transfer, general
Right arrow Viral Methods
Right arrow Cell Biology, general
Right arrow Immunology, general
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Epstein-Barr Virus Transformation of Lymphoblasts

Gretchen J. Darlington

This protocol was adapted from "Isolation and Transformation of Lymphocytes," Chapter 7, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998. This three-volume set is now out of print; however, some of the microscopy methods were republished in Basic Methods in Microscopy, by David L. Spector and Robert D. Goldman.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for the transformation of lymphoblasts by Epstein-Barr Virus (EBV). Cells may be isolated from whole blood or taken from cryopreserved, non-immortalized stocks (please see Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples). The source of infectious EBV is the conditioned medium from marmoset cell line B95-8.


MATERIALS

Reagents

Cyclosporin A (Sigma)

caution Whole blood

caution EBV-containing supernatant

EBV-containing supernatant is derived from the . . . [Full Text of this Article]

Equipment:


METHOD


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Protocol

Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples
Gretchen J. Darlington
CSH Protocols 2006: 4350. [Extract] [Full Text]