Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4459

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Static Culture of Postimplantation Embryos for Imaging

Mary Dickinson, Elizabeth Jones, David Crotty, and Scott Fraser

Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer

This protocol was adapted from "Isolation, Culture, and Manipulation of Postimplantation Embryos," Chapter 5 (Protocol 9, procedure provided by Mary Dickinson, Elizabeth Jones, David Crotty and Scott Fraser), in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for static culture of early postimplantation mouse embryos on a microscope stage. Embryos between 6.5 and 9.5 days post coitum (dpc) can be cultured and imaged for 24 hours, with very little growth retardation.


MATERIALS

Reagents

recipe Dissection medium

Ethanol, 70%

Gas mixture: 5% CO2, balance air

Hair

Mineral oil (Sigma)

Mouse Embryos (6.5-9.5 dpc) (see Isolating Postimplantation Embryos: Early Primitive-Streak-Stage, Isolating Postimplantation Embryos: Late Primitive-Streak-Stage, Isolating Postimplantation Embryos: Early Neural-Fold-Stage, Isolating Postimplantation Embryos: Early Somite-Stage, depending on age of embryos used)

recipe . . . [Full Text of this Article]

Equipment


METHOD


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