Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4452

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Storage of Bacterial Cultures Growing in Liquid Media

Joseph Sambrook and David W. Russell

This protocol was adapted from "Commonly Used Techniques in Molecular Cloning," Appendix 8, in Molecular Cloning, Volume 3, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for storing bacterial cultures containing glycerol which are growing in liquid media.


MATERIALS

Reagents

Bacterial culture growing in appropriate liquid medium

caution caution Ethanol-dry ice orliquid . . . [Full Text of this Article]

Equipment


METHOD


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