Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4430

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Analysis of DNA Fragmentation Using Propidium Iodide (PI) Fluorescence of Individual Nuclei

Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green

This protocol was adapted from "Apoptosis Assays," Chapter 15, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998. This three-volume set is now out of print; however, some of the microscopy methods were republished in Basic Methods in Microscopy, by David L. Spector and Robert D. Goldman.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol provides a method for analysis of DNA fragmentation using flow cytometry after propidium iodide (PI) labeling of apoptotic nuclei. Apoptotic nuclei stain less than their normal counterparts because of diffusion of low-molecular-weight fragments out of the cells and/or chromatin condensation that . . . [Full Text of this Article]


MATERIALS

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METHOD


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