Protocol

Introduction of Double-Stranded RNA in C. elegans by Injection

This protocol was adapted from "RNAi in C. elegans," contributed by René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk, Chapter 4, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.

INTRODUCTION

Double-stranded RNA (dsRNA) can be efficiently introduced into Caenorhabditis elegans by microinjection into the gonad, the gut, or the body fluid. The RNAi effect will spread within the nematode, exerting an effect beyond the site of injection. Alternative methods for introducing dsRNA are soaking the animals in a solution of dsRNA (see Introduction of Double-Stranded RNA in C. . . . [Full Text of this Article]

MATERIALS

Reagents

Equipment

METHOD

Troubleshooting