Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4224

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Extraction and Solubilization of Total Protein from Microorganisms

Angelika Görg, Oliver Drews, and Walter Weiss

This protocol was adapted from "Extraction and Solubilization of Cell and Tissue Samples," contributed by Angelika Görg, Oliver Drews, and Walter Weiss, Chapter 16, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

The first 15% of the full text of this article appears below.


INTRODUCTION

The growth conditions of microbial cell cultures and the time of sample collection should be optimized and standardized when growing cells for protein extraction. Because cells may excrete proteases and other extracellular enzymes, and compounds in the medium may interfere with extraction, wash cultures with an isotonic buffer, such as PBS or sucrose before solubilization. To avoid stress induction, the temperature and . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


TROUBLESHOOTING


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