Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4195

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Westerlund, B.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Westerlund, B.
Related Collections
Right arrow Molecular Biology, general
Right arrow Proteins and Proteomics, general
Right arrow Chromatography, general
Right arrow Ion-Exchange Chromatography
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Preparation of an Ion-Exchange Column

Bengt Westerlund

This protocol was adapted from "Ion-Exchange Chromatography," contributed by Bengt Westerlund, Chapter 6, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

The first 15% of the full text of this article appears below.


INTRODUCTION

Ion-exchange chromatography (IEC) can be used as a crude step in a protein purification scheme, or, with proper preparation, as a high-resolution step. If high resolution is desired, considerable care should be taken during column preparation, choice of IEC media, and column packing.


MATERIALS

Reagents

Buffer (see Tables 1, 2, and 3)

The buffer used in this protocol should be the same as the binding buffer that will be used when protein is applied to the column. . . . [Full Text of this Article]

Equipment


METHOD


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?