Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4194

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Selection of an Ion Exchanger: Determining the pI of a Protein Using the Trial-and-Error Method

This protocol was adapted from "Ion-Exchange Chromatography," contributed by Bengt Westerlund, Chapter 6, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

The first 15% of the full text of this article appears below.


INTRODUCTION

The choice of whether to use an anion or a cation exchanger should be based on knowledge of the stability of the protein, and the binding properties of the target protein and other molecules present in the sample. The isoelectric point (pI) of a protein has a great bearing on its behavior and . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


TROUBLESHOOTING


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