Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4156

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Hydroxylamine Mutagenesis of Plasmid DNA

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Hydroxylamine Mutagenesis of Plasmid DNA," in Techniques and Protocols 7, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol produces mutagenized DNA that can be used for transformation of either Escherichia coli or yeast.


MATERIALS

Reagents

recipe Bovine serum albumin (BSA), 1 mg/ml

Plasmid DNA, CsCl-purified (10 µg) (see Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients: Discontinuous Gradients)

Ethanol

recipe caution Hydroxylamine mutagenesis solution, . . . [Full Text of this Article]

Equipment


METHOD


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Related Protocol

Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients: Discontinuous Gradients
Joseph Sambrook and David W. Russell
CSH Protocols 2006: 3913. [Extract] [Full Text]