Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4149

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Isolation of Yeast Genomic DNA for Southern Blot Analysis

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Yeast DNA Isolations," Techniques and Protocols 3, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol (modified from Hoffman and Winston 1987) describes the purification of genomic DNA from yeast cells. It is an adaptation of Isolation of Plasmid DNA from Yeast Cells: A Ten-Minute Preparation.


MATERIALS

Reagents

recipe Ammonium acetate, 4 M

Appropriate yeast culture

Ethanol

caution Phenol:chloroform:isoamyl alcohol (25:24:1)

recipe RNase A in potassium acetate . . . [Full Text of this Article]

Equipment


METHOD


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