Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4148

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Yeast DNA Isolation: Miniprep

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Yeast DNA Isolations," Techniques and Protocols 3, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

There are numerous methods available for the isolation of yeast DNA. This protocol describes the preparation of DNA from a 5-ml liquid culture of yeast.


MATERIALS

Reagents

Appropriate yeast culture

Isopropanol

recipe Potassium acetate, 5 M

recipe RNase A in potassium acetate solution (1 mg/ml) (optional, see Step 13)

recipe SDS, 10%

recipe Sodium acetate, 3 M

Sorbitol, 1 M containing 0.1 M Na2 EDTA (pH 7.5)

recipe TE (pH . . . [Full Text of this Article]

Equipment


METHOD


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