Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4147

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Yeast DNA Isolation: Midiprep

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Yeast DNA Isolations," Techniques and Protocols 3, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

There are numerous methods for the isolation of DNA from yeast cells. This protocol describes the preparation of DNA from a 40-ml liquid culture.


MATERIALS

Reagents

Appropriate yeast culture

Ethanol, 95%

Isopropanol

recipe Potassium acetate, 5 M

recipe RNase A in potassium acetate solution (1 mg/ml)

recipe SDS, 10%

Sorbitol, 0.9 M, containing 0.1 M Na2 EDTA (pH 7.5)

recipe TE (pH 7.4)

Tris-Cl, 50 mM (pH 7.4) containing 20 mM Na2 EDTA

recipe YPD . . . [Full Text of this Article]

Equipment


METHOD


TROUBLESHOOTING


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