Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4146

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"Quick and Dirty" Plasmid Transformation of Yeast Colonies

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "‘Quick and Dirty’ Plasmid Transformation of Yeast Colonies," Techniques and Protocols 2, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

Several methods exist for yeast transformation. This protocol can be used if high efficiency is not needed. It is modified from Chen et al. (1992). For higher efficiency transformation, High-Efficiency Transformation of Yeast is recommended.


MATERIALS

Reagents

Appropriate . . . [Full Text of this Article]

Equipment


METHOD


TROUBLESHOOTING


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