Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4145

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High-Efficiency Transformation of Yeast

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "High-Efficiency Transformation of Yeast," Techniques and Protocols 1, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 100 words of the full text of this article appear below.


INTRODUCTION

Several methods exist for yeast transformation. This protocol describes high-efficiency transformation and was adapted from Gietz and Schiestl (1995). A simpler, though less efficient, transformation protocol can be found in "Quick and Dirty" Plasmid Transformation of Yeast Colonies.


MATERIALS

Reagents

Appropriate plates containing selective media

High-molecular-weight DNA (deoxyribonucleic acid sodium salt type III from salmon testes; Sigma)

recipe caution Lithium acetate, 1 M and 100 mM

Plasmid DNA for transformation

Polyethylene glycol (PEG) 3350 (Sigma), 50% (w/v)

recipe TE buffer (pH 8.0)

Yeast strain to be transformed

recipe recipe YPAD, liquid or synthetic complete (SC) medium

Equipment

Boiling water bath, preset to 100°C

Shaking incubator, preset to . . . [Full Text of this Article]


METHOD


TROUBLESHOOTING


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Related Protocol

"Quick and Dirty" Plasmid Transformation of Yeast Colonies
David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern
CSH Protocols 2006: 4146. [Extract] [Full Text]