Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4096

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, C.
Right arrow Articles by Shiels, G.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Smith, C.
Right arrow Articles by Shiels, G.
Related Collections
Right arrow Molecular Biology, general
Right arrow Genomic DNA
Right arrow Polymerase Chain Reaction (PCR), general
Right arrow Purification for PCR
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Purification of Genomic DNA from Whole Blood: A Solution-Based Method

Craig Smith, Paul Otto, Rex Bitner, and Gary Shiels

This protocol was adapted from "DNA Purification," contributed by Craig Smith, Paul Otto, Rex Bitner, and Gary Shiels in Chapter 9, PCR Primer, 2nd edition (eds. Dieffenbach and Dveksler). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, 2003.

The first 100 words of the full text of this article appear below.


INTRODUCTION

The isolation of genomic DNA from whole blood (10 ml) using the Wizard Genomic DNA Purification Kit is based on a four-step process: lysis of the red blood cells and removal of the soluble fraction; lysis of the white blood cells and their nuclei; selective removal of cellular proteins; and the concentration and desalting of the purified genomic DNA. The Wizard Genomic DNA Purification Kit protocol can be used to isolate genomic DNA from whole-blood samples between 20 µl and 10 ml of whole blood collected in EDTA-, heparin-, or citrate-coated tubes.


MATERIALS

Reagents

Ethanol, 70%, room temperature

Isopropanol, room temperature

recipe RNase . . . [Full Text of this Article]

Equipment


METHOD


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Protocol

Agarose Gel Electrophoresis
Joseph Sambrook and David W. Russell
CSH Protocols 2006: 4020. [Extract] [Full Text]