Please cite as: CSH Protocols; 2008; doi:10.1101/pdb.prot4941

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Fixation, Embedding, and Sectioning of Plant Tissues

Detlef Weigel and Jane Glazebrook

This protocol was adapted from "How to Study Gene Expression," Chapter 7, in Arabidopsis, by Detlef Weigel and Jane Glazebrook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

This protocol describes the preparation of plant tissues for sectioning and subsequent use in immunohistochemistry or in situ hybridization. Tissues of interest are fixed and embedded in Paraplast before sectioning.


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