Please cite as: CSH Protocols; 2008; doi:10.1101/pdb.prot4917

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Collection of Drosophila Embryos for RNA Interference (RNAi)

Leonie Misquitta, Qin Wei, and Bruce M. Paterson

This protocol was adapted from "Targeted Disruption of Gene Function in Drosophila by RNA Interference," Chapter 19, in Drosophila Protocols (eds. Sullivan et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000. Please note that this version of the protocol is a 2007 revision.


INTRODUCTION

RNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. This protocol describes a method for collection of Drosophila embryos for RNA interference (RNAi) experiments. The embryos are collected in a simple, homemade apparatus, arrayed on prepared glass slides, and readied for injection. It is important to keep the embryos moist and oxygenated. Work expeditiously, because embryos should be injected within 30-60 min after collection.


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Drosophila RNA Interference (RNAi) Using a Gal-4 Inducible Transgene Vector
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