Please cite as: CSH Protocols; 2008; doi:10.1101/pdb.prot4925

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Transfecting Neural Crest Cells in Avian Embryos Using In Ovo Electroporation

Stephanie R. Kadison and Catherine E. Krull1

Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA

1Corresponding author (krullc{at}umich.edu)


INTRODUCTION

This protocol outlines a method for introducing genes into the neural crest cells of avian embryos at vagal/cardiac and trunk levels. Electroporation offers the unique opportunity to misexpress genes or "knock down" the expression of their protein products using short hairpin RNAs (shRNAs) within targeted populations of cells. Electroporated cells can be visualized by using DNA plasmids that contain enhanced green fluorescent protein (EGFP) or other fluorescent reporter proteins.


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This article has been cited by other articles:


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 CSHL ProtocolsHome page
Y.-X. Chen and C. E. Krull
Using In Ovo Electroporation to Transfect Cells in Avian Somites
CSH Protocols, January 1, 2008; 2008(2): pdb.prot4924 - pdb.prot4924.
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 CSHL ProtocolsHome page
S. A. Linn and C. E. Krull
Transfecting Avian Motor Neurons and Their Axons Using In Ovo Electroporation
CSH Protocols, January 1, 2008; 2008(2): pdb.prot4926 - pdb.prot4926.
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