Please cite as: CSH Protocols; 2008; doi:10.1101/pdb.prot4887

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Cell Transfection and Analysis Using DNA-Loaded Gelatin Nanoparticles

Sushma Kommareddy and Mansoor M. Amiji

This protocol was adapted from "Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles," Chapter 52, in Gene Transfer: Delivery and Expression of DNA and RNA (eds. Friedmann and Rossi). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2007.


INTRODUCTION

This protocol describes cell-culture techniques for transfection using DNA-loaded gelatin nanoparticles. Also included are methods for qualitative analysis of transfection with fluorescence microscopy and quantitative analysis using a fluorescence-activated cell sorter (FACS).


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