Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4795

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Fixation of Imaginal Discs in Drosophila

Seth S. Blair

This protocol was adapted from "Imaginal Discs," Chapter 10, in Drosophila Protocols (eds. Sullivan et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000.


INTRODUCTION

Imaginal disc primordia appear in Drosophila embryos as clusters of cells that invaginate from the embryonic epithelium. During metamorphosis, the imaginal discs form much of the outer covering of the developing adult. Generation of genetic mosaics is useful for removing or adding gene function to imaginal cells. This protocol describes the methods for fixing such tissues in preparation for immunohistological techniques to monitor gene expression.


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