Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4872

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Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis

Amber L. Wells1, John S. Condeelis1,2, Robert H. Singer1,2,3, and Daniel Zenklusen1

1 Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
2 Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA

3Corresponding author (rhsinger{at}aecom.yu.edu)


INTRODUCTION

This protocol describes the use of ImageJ software (freely available from NIH) to analyze particle dynamics in a cell using time-lapse movie frames or image stacks of fluorescent mRNA particles. Maximum intensity projections and kymographs are produced.


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