Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4867

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Constructing Sample Chambers for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

Paul R. Selvin1, Tyler Lougheed, Melinda Tonks Hoffman, Hyokeun Park, Hamza Balci, Benjamin H. Blehm, and Erdal Toprak

Biophysics Center and Physics Department, University of Illinois, Urbana, IL 61801, USA

1Corresponding author (selvin{at}uiuc.edu)


INTRODUCTION

FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This protocol provides details on constructing an inexpensive sample chamber for use in single-molecule FIONA experiments and two methods for cleaning slides and coverslips.


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