Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4848

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fuller, L.
Right arrow Articles by Dailey, M. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Fuller, L.
Right arrow Articles by Dailey, M. E.
Related Collections
Right arrow Cell Biology, general
Right arrow Visualization
Right arrow Visualization, general
Right arrow Imaging/Microscopy, general
Right arrow Laboratory Organisms, general
Right arrow Mouse
Right arrow Other Laboratory Organisms
Right arrow Neuroscience, general
Right arrow Neural Cell Culture
Right arrow Developmental Biology
Right arrow Explant Culture
Right arrow Cell Imaging
Right arrow Imaging for Neuroscience
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Preparation of Rodent Hippocampal Slice Cultures

Leah Fuller and Michael E. Dailey1

Department of Biology, University of Iowa, Iowa City, IA 52242, USA.

1Corresponding author (michael-e-dailey{at}uiowa.edu)


INTRODUCTION

Rodent organotypic hippocampal slice cultures (OHSCs) provide an outstanding preparation of central nervous system tissue for exploring the dynamic structural and physiological features of neuronal and glial cells within their native three-dimensional environments. It is a straightforward matter to obtain tissue slices from neonatal rodents. These slices culture well for periods up to several weeks and are easy to manipulate, allowing for a variety of in vitro experimental models. OHSCs provide good optical and physiological accessibility for studies involving live cell imaging, with high spatial and temporal resolution. This protocol is used to harvest tissues for both immunohistochemical labeling after fixation, and for confocal time-lapse imaging in live tissues labeled by a variety of fluorescent dyes or by biolistic or viral transfection.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?