Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4800

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Observational Methods Used to Assess Rat Behavior: Behavioral Patterns

Carol Ann Paul, Barbara Beltz, and Joanne Berger-Sweeney

This protocol was adapted from "Neurons Coordinating Behavior," Section III, in Discovering Neurons: The Experimental Basis of Neuroscience (eds. Paul et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1997.

[Supplemental Material is available online at www.cshprotocols.org/supplemental/.]

INTRODUCTION

This protocol describes a method for quantifying observation of normal rat behaviors in a controlled environment and in a defined area. It is necessary to have precise definitions of the behaviors to be quantified and a reliable procedure for quantifying them. The observation room should be quiet, and it should be equipped for light or dark observations (rats cannot see red light so dark observations can be made using a red light). Ideally, the observer should be separated from the animal by a one-way screen. If such a screen is not available, the observer should stand or sit in a constant, unobtrusive position and make as few movements as possible.


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