Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4831

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Preparing DNA from Saliva for Genotyping

Amrik Sahota1,3, Andrew I. Brooks1,2, and Jay A. Tischfield1

1Rutgers University Cell and DNA Repository, Department of Genetics, Piscataway, NJ 08854-8082, USA
2Bionomics Research and Technology Center, Environmental and Occupational Health Sciences Institute, University of Medicine and Dentistry of New Jersey--Robert Wood Johnson Medical School, Piscataway, NJ 08854-5635, USA

3Corresponding author (sahota{at}biology.rutgers.edu)


INTRODUCTION

Saliva may be a viable alternative to blood as a source for DNA, and has the advantage that it is collected noninvasively. This protocol describes the Oragene procedure for DNA extraction from 2-mL saliva samples. It yields high-quality DNA suitable for research and diagnostic applications.


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Related Article

Preparing DNA from Mammalian Sources for Genotyping
Amrik Sahota, Andrew I. Brooks, and Jay A. Tischfield
CSH Protocols 2007: 19. [Abstract] [Full Text]