Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4829

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Preparing DNA from Cell Pellets for Genotyping

Amrik Sahota1,3, Andrew I. Brooks1,2, and Jay A. Tischfield1

1Rutgers University Cell and DNA Repository, Department of Genetics, Piscataway, NJ 08854-8082, USA
2Bionomics Research and Technology Center, Environmental and Occupational Health Sciences Institute, University of Medicine and Dentistry of New Jersey--Robert Wood Johnson Medical School, Piscataway, NJ 08854-5635, USA

3Corresponding author (sahota{at}biology.rutgers.edu)


INTRODUCTION

This protocol describes large-scale DNA extraction from cell pellets using either in-house or commercial (PUREGENE) reagents. The cell pellets are prepared from human lymphoblast cell lines (LCLs) that have been established using the Epstein-Barr virus (EBV).


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Preparing DNA from Mammalian Sources for Genotyping
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