Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4779

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Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes

Rosa A. Uribe1,2 and Jeffrey M. Gross1,2,3,4

1 Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, Austin, Texas 78712, USA
2 Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA
3 Institute for Neuroscience, The University of Texas at Austin, Austin, Texas 78712, USA

4Corresponding author (jmgross{at}mail.utexas.edu)


INTRODUCTION

This protocol describes methods for the fixation, cryosectioning, and immunohistochemical detection of proteins in embryonic and adult zebrafish eyes. The methods described can easily be adapted for use on other zebrafish tissues.


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R. J. Nuckels and J. M. Gross
Histological Preparation of Embryonic and Adult Zebrafish Eyes
CSH Protocols, September 1, 2007; 2007(18): pdb.prot4846 - pdb.prot4846.
[Abstract] [Full Text]