Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4702

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Fixation of Mouse Embryos and Tissues

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems," Chapter 16, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

This protocol provides methods for fixation of mouse embryos and tissues with paraformaldehyde, Bouin’s fixative, or methanol/DMSO solution.


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