Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4623

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Setting Up Arabidopsis Crosses

Detlef Weigel and Jane Glazebrook

This protocol was adapted from "Genetic Analysis of Mutants," Chapter 3 in Arabidopsis by Detlef Weigel and Jane Glazebrook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

The inheritance of mutant phenotypes is most often analyzed in the progeny resulting from crosses between different parents. Because Arabidopsis naturally self-pollinates, the generation of cross-progeny requires some intervention by the investigator. This protocol describes the generation and collection of seeds by crossing suitable Arabidopsis parent plants.


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