Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4553

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Analysis of Complex Protein Mixtures Using Nano-LC Coupled to MS/MS

David M. Schieltz, Michael P. Washburn, and Lara G. Hays

This protocol was adapted from "The Use of Mass Spectrometry in Proteomics," Chapter 8 in Proteins and Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

Nanoliter-LC coupled to tandem mass spectrometry (nano-LC-MS/MS) permits the rapid and sensitive determination of protein-protein interactions. By using a specific purification technique such as coimmunoprecipitation or affinity purification in conjunction with nano-LC-MS/MS, not only are proteins identified, but specific protein-protein interactions are elucidated as well.


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