Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4506

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Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos

Esther T. Stoeckli

This protocol was adapted from "RNAi in Avian Embryos," contributed by Esther T. Stoeckli, Chapter 14, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

This protocol describes a method for staining nerve fibers in whole-mount preparations of avian embryos using an antibody against the 160-kD subunit of neurofilaments. This allows the comparison of the branching pattern of motor and sensory neurons between control and experimental embryos. This protocol has been successfully applied for embryos at different stages up to about stage 33 (7 days of incubation).


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