Please cite as: CSH Protocols; 2008; doi:10.1101/pdb.prot4987

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Google Scholar
Right arrow Articles by Fischer, A. H.
Right arrow Articles by Zeller, R.
PubMed
Right arrow Articles by Fischer, A. H.
Right arrow Articles by Zeller, R.
Related Collections
Right arrow Purifying Antibodies
Right arrow Cell Imaging
Right arrow Cell Biology, general
Right arrow Visualization
Right arrow Imaging/Microscopy, general
Right arrow Light Microscopy
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Cutting Sections of Paraffin-Embedded Tissues

Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller

This protocol was adapted from "Preparation of Cells and Tissues for Fluorescence Microscopy," Chapter 4, in Basic Methods in Microscopy (eds. Spector and Goldman). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2006.


INTRODUCTION

This protocol describes the sectioning of tissues embedded in paraffin blocks. Paraffin sections require extensive fixation and processing steps but provide superior morphology compared with other sectioning methods. Sectioning paraffin blocks requires experience and should be learned from an experienced researcher, if possible.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Protocols

Preparation of Slides and Coverslips for Microscopy
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols 2008: 4988. [Abstract] [Full Text]

Paraffin Embedding Tissue Samples for Sectioning
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols 2008: 4989. [Abstract] [Full Text]

Decalcifying Tissues for Paraffin Embedding
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols 2008: 4990. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 CSHL ProtocolsHome page
A. H. Fischer, K. A. Jacobson, J. Rose, and R. Zeller
Hematoxylin and Eosin Staining of Tissue and Cell Sections
CSH Protocols, May 1, 2008; 2008(6): pdb.prot4986 - pdb.prot4986.
[Abstract] [Full Text]

Home page
 CSHL ProtocolsHome page
A. H. Fischer, K. A. Jacobson, J. Rose, and R. Zeller
Paraffin Embedding Tissue Samples for Sectioning
CSH Protocols, May 1, 2008; 2008(6): pdb.prot4989 - pdb.prot4989.
[Abstract] [Full Text]

Home page
 CSHL ProtocolsHome page
A. H. Fischer, K. A. Jacobson, J. Rose, and R. Zeller
Decalcifying Tissues for Paraffin Embedding
CSH Protocols, May 1, 2008; 2008(6): pdb.prot4990 - pdb.prot4990.
[Abstract] [Full Text]