Please cite as: CSH Protocols; 2008; doi:10.1101/pdb.prot4883

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Google Scholar
Right arrow Articles by Cornetta, K.
Right arrow Articles by Miller, A. D.
PubMed
Right arrow Articles by Cornetta, K.
Right arrow Articles by Miller, A. D.
Related Collections
Right arrow Virus
Right arrow Molecular Biology, general
Right arrow Vectors
Right arrow Transgenic Technology, general
Right arrow DNA Delivery/Gene Transfer
Right arrow DNA Delivery/Gene Transfer, general
Right arrow Viral Methods
Right arrow Cell Biology, general
Right arrow Cell Culture
Right arrow Preparation of Macromolecules and Introduction into Cells
Right arrow Labeling for Imaging
Right arrow Laboratory Organisms, general
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Transduction of Cell Lines by Retroviral Vectors

Kenneth Cornetta, Karen E. Pollok, and A. Dusty Miller

This protocol was adapted from "Retroviral Vectors," Chapter 2, in Gene Transfer: Delivery and Expression of DNA and RNA (eds. Friedmann and Rossi). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2007.


INTRODUCTION

This protocol is suitable for transduction of many adherent cell lines. The number of target cells transduced can be varied as needed by maintaining the ratio of surface area to volume and using plates/flasks of various sizes. The protocol can also easily be adapted for non-adherent cells using similar vector-to-cell ratios.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Protocols

Retroviral Vector Production by Transient Transfection
Kenneth Cornetta, Karen E. Pollok, and A. Dusty Miller
CSH Protocols 2008: 4881. [Abstract] [Full Text]

Generation of Stable Vector-Producing Cells for Retroviral Vectors
Kenneth Cornetta, Karen E. Pollok, and A. Dusty Miller
CSH Protocols 2008: 4882. [Abstract] [Full Text]