Please cite as: CSH Protocols; 2008; doi:10.1101/pdb.prot4936

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Vital Stain for Plant Cytoplasm

Birgit Schwab and Martin Hülskamp

This protocol was adapted from "How to Analyze a Mutant Phenotypically," Chapter 4, in Arabidopsis (eds. Weigel and Glazebrook). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

This protocol describes a vital stain for plant cytoplasm. Fluorescein diacetate is used to identify viable cells, which will emit a greenish fluorescence. Dead cells will fluoresce red in the presence of propidium iodide, which does not enter live cells.


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