Please cite as: CSH Protocols; 2008; doi:10.1101/pdb.ip52

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Media for Mounting Fixed Cells on Microscope Slides

Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller

Adapted from "Preparation of Cells and Tissues for Fluorescence Microscopy," Chapter 4, in Basic Methods in Microscopy (eds. Spector and Goldman). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2006.


INTRODUCTION

After a specimen is labeled, coverslips containing cells or tissues are mounted onto microscope slides, or slides containing sections are overlaid with a coverslip. A number of recipes for commonly used mounting media are presented in this article, each with particular recommendations.


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