Please cite as: CSH Protocols; 2008; doi:10.1101/pdb.prot4918

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Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)

Leonie Misquitta, Qin Wei, and Bruce M. Paterson

This protocol was adapted from "Targeted Disruption of Gene Function in Drosophila by RNA Interference," Chapter 19, in Drosophila Protocols (eds. Sullivan et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000. Please note that this version of the protocol is a 2007 revision.


INTRODUCTION

RNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. This protocol describes a technique by which Drosophila embryos can be injected with dsRNA in order to disrupt targeted gene function. The approach is straightforward, utilizing improved methods for injecting the dsRNA directly through the chorion of the embryo. This strategy minimizes problems normally associated with desiccation of the dechorionated embryo and facilitates post-injection analysis of gene expression.


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