Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.top5

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topic_introductionTopic Introduction

Quantitative Microinjection of Drosophila Embryos: General Strategy

Daniel P. Kiehart, Janice M. Crawford, and Ruth A. Montague

Adapted from "Quantitative Microinjection of Drosophila Embryos," Chapter 18, in Drosophila Protocols (eds. Sullivan et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000.


INTRODUCTION

Microinjection of Drosophila embryos is a common technique used by a wide range of investigators, but some applications require a refined strategy for handling embryos. This article outlines the general procedures for microinjection and quantification of aqueous solutions during high-resolution observation of early development in the fly embryo. It also describes the design of suitable support slides for the manipulation of Drosophila embryos under upright and inverted microscopes.


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Related Protocols

Collection, Dechorionation, and Preparation of Drosophila Embryos for Quantitative Microinjection
Daniel P. Kiehart, Janice M. Crawford, and Ruth A. Montague
CSH Protocols 2007: 4717. [Abstract] [Full Text]

Quantitative Microinjection of Drosophila Embryos
Daniel P. Kiehart, Janice M. Crawford, and Ruth A. Montague
CSH Protocols 2007: 4718. [Abstract] [Full Text]



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