Protocol

Imaging X-gal-Stained Mouse Embryos

This protocol was adapted from "Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems," Chapter 16, in Manipulating the Mouse Embryo, 3rd edition (eds. Nagy et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

INTRODUCTION

Following staining for ß-galactosidase (lacZ) activity, whole mouse embryos are postfixed in paraformaldehyde, placed on a bed of agar, and visualized using bright- or dark-field microscopy.