Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4719

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Staining Proteins in Gels with Coomassie Blue

Richard J. Simpson

This protocol was adapted from "Peptide Mapping and Sequence Analysis of Gel-Resolved Proteins," Chapter 7, in Proteins and Proteomics by Richard J. Simpson. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

The most commonly used dye for visualizing proteins in SDS-PAGE gels is Coomassie Brilliant Blue R250 (CBR-250) because of its relatively high sensitivity. This protocol describes the standard CBR-250 staining method, along with a simple method for preparing stained gels for long-term storage.


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