Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.ip12
| Information Panel |
This information panel was adapted from "Protein Interactions Captured by Chemical Cross-linking," Chapter 7, in Protein-Protein Interactions (eds. Golemis and Adams). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.
| The first 15% of the full text of this article appears below. |
The final step in a protein cross-linking reaction is quenching. Excess amounts of nucleophiles (e.g., glycine, Tris) or other reagents that compete for the reactive groups of the
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