Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4697

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Cleavage of Asn-Gly Bonds by Hydroxylamine

Richard J. Simpson

This protocol was adapted from "Peptide Mapping and Sequence Analysis of Gel-Resolved Proteins," Chapter 7, in Proteins and Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

Selective cleavage of Asn-Gly bonds in proteins by hydroxylamine generates a relatively small number of large peptide fragments. This protocol describes a procedure for performing Asn-Gly bond cleavage in solution.


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