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Live-Cell Imaging of GFP in Plants

Adapted from "How to Study Gene Expression," Chapter 7, in Arabidopsis: A Laboratory Manual (eds. Weigel and Glazebrook). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.

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Green Fluorescent Protein

The GFP from jellyfish Aequorea victoria is a very stable and relatively small protein of 238 amino acids.

GFP serves as a molecular marker that can be imaged dynamically in living cells, both in its native form and as a fusion to other proteins. For GFP imaging, plants present the challenge of autofluorescence from chlorophyll, lignified cell walls, vacuolar contents, and other cell materials, all of which can obscure the GFP signal. Maximizing the signal-to-noise ratio is a major concern, and careful consideration should be given to the choice of tissue imaged, GFP expression level, GFP spectral variant (e.g., mGFP5), . . . [Full Text of this Article]

Construct Design

GFP Imaging

Imaging of Roots

Imaging of Primordia on the Shoot Apex

Imaging of Hypocotyls and Leaves