Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.ip28

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Imaging Actin in Tissue Slices from Transgenic Mouse Brain

Andrew Matus, Virginie Biou, Heike Brinkhaus, and Martijn Roelandse

This information panel was adapted from "Imaging the Actin Cytoskeleton," Chapter 29, in Live Cell Imaging (eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.

The same GFP-tagged actin construct used in cell transfection experiments has been used to produce transgenic mice. Transgenic animals allow the imaging of brain tissue in the intact animal, as acutely cut slices or as organotypic slice cultures. They also serve as a source of cells for imaging neurons at high resolution in dispersed low-density cell culture. In contrast to cells transfected in culture, where the level of actin-GFP expression in neurons varies considerably, transgenic mice provide reproducibly labeled cells and tissues. Imaging GFP-tagged proteins in brain tissue requires confocal . . . [Full Text of this Article]

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