Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.top28

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topic_introductionTopic Introduction

Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Construct Design and Imaging System Setup

Amber L. Wells1, John S. Condeelis1,2, Robert H. Singer1,2,3, and Daniel Zenklusen1

1 Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
2 Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA

3Corresponding author (rhsinger{at}aecom.yu.edu)


INTRODUCTION

The most common way for a cell to respond to internal and external signals is to change its gene expression pattern. This requires the synchronization of regulatory steps along the expression pathway. Biological imaging techniques can be used to visualize and measure such processes in individual live cells in real time. This article discusses the use of a fluorescent RNA-binding protein system that allows real-time analysis of gene expression with single-transcript resolution.


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