Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.ip46

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Data Analysis Methods for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

Paul R. Selvin1, Tyler Lougheed, Melinda Tonks Hoffman, Hyokeun Park, Hamza Balci, Benjamin H. Blehm, and Erdal Toprak

Biophysics Center and Physics Department, University of Illinois, Urbana, IL, 61801, USA

1Corresponding author (selvin{at}uiuc.edu)


INTRODUCTION

FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. Data analysis of a FIONA experiment requires the use of several software programs. Data must be acquired and exported into a proper format before analysis can take place. This article describes various options for data analysis.


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