Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.ip45

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Equipment Setup for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

Paul R. Selvin1, Tyler Lougheed, Melinda Tonks Hoffman, Hyokeun Park, Hamza Balci, Benjamin H. Blehm, and Erdal Toprak

Biophysics Center and Physics Department, University of Illinois, Urbana, IL 61801, USA

1Corresponding author (selvin{at}uiuc.edu)


INTRODUCTION

FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This article describes procedures for setting up the equipment necessary for FIONA and achieving total internal reflection (TIR).


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